The Facilities Integrating Collaborations for User Science (FICUS) Call for Proposals accelerates ambitious user projects by providing access to world-class resources at multiple facilities through a single user proposal. This FICUS Call for Proposals provides access to advanced molecular characterization capabilities at the Center for Structural Molecular Biology and the Environmental Molecular Sciences Laboratory (EMSL); state-of-the-art sequencing, synthesis, metabolomics, and data analysis at the Joint Genome Institute (JGI); and continental-scale remote sensing measurement infrastructure through the National Ecological Observatory Network (NEON).